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Transfer efficiencies of 80–100% are achievable for proteins between 14–116 kDa.

A high field option exists for a single gel, which may bring transfer time down to as little as 30 minutes, but it requires the use of high voltage (up to 200 V) or high current (up to 1.6 A) and a cooling system to dissipate the tremendous heat produced. Transfers are typically performed with an ice pack and at 4☌ to mitigate the heat produced. Multiple gels may be electrotransferred in the standard field option, which is performed either at constant current (0.1 to 1 A) or voltage (5 to 30 V) from as little as 1 hour to overnight. The supported gel sandwich is placed vertically in a tank between stainless steel/platinum wire electrodes and filled with transfer buffer. The gel is then placed in the “transfer sandwich” (filter paper-gel-membrane-filter paper), cushioned by pads and pressed together by a support grid. When performing a wet transfer, the gel is first equilibrated in transfer buffer. SureLock Tandem Midi Gel Tank: capacity for up to 2 blot modules XCell SureLock Mini-Cell: capacity for up to 2 blot modules Mini Gel Tank: capacity for up to 2 Mini Blot Modules Pre-assembled Select transfer stacks: buffer not required 1 mini gel per blot module 1–2 blot modules per tankġ midi gel per blot module 1–2 blot modules per tank